카테고리 보관물: Fluorescence

EtBr

Spectrum Chart

EtBr Spectrum
Excitation () Emission ()
520 603

 

Recommended Filter set for for EtBr

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39009 480/30
(465~495)
505 (LP) 620/60
(590~650)
Medium Excitation band,
Bandpass Emission
49005 545/30
(530~560)
570 (LP) 620/60
(590~650)
Medium Excitation band,
Bandpass Emission

  • 레이저 Lighting source -> 문의 주시기 바랍니다.

 

 

Information

As with most fluorescent compounds, ethidium bromide is aromatic. Its core heterocyclic moiety is generically known as a phenanthridine, an isomer of which is the fluorescent dye acridine. Absorption maxima of EtBr in aqueous solution are at 210 and 285 , which correspond to ultraviolet light. As a result of this excitation, EtBr emits orange light with wavelength 605 .

대부분의 형광 화합물과 마찬가지로 에티듐 브로마이드는 방향족으로  중심 헤테로시 클릭 잔기는 페난 트리 딘으로 일반적으로 알려져 있으며, 이의 이성질체는 형광 염료 아 크리 딘입니다. 수용액에서의 EtBr의 흡수 최대 값은 210 ㎚ 와 285 ㎚ 에서 자외선에 해당하며 이 여기의 결과로 EtBr은 파장 605 ㎚ 의 주황색 광을 방출합니다.

 

 

Cy7

Spectrum Chart

Cy7 Spectrum
Excitation () Emission ()
743 767

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49007 710/75
(672.5~747.5)
760 (LP) 810/90
(765~855)
Very Wide Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source -> 문의 주시기 바랍니다.

Information

Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV.

Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.

Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 ). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

Cyanines(Cy) replace advantageously conventional dyes such as Fluorescein(FITC) and rhodamines (TRITC, RRX), yielding brighter and more stable fluorescence.

시아닌은 여전히 업계에서, 그리고 최근에는 생명 공학 (라벨링, 분석)에서 사용되고 있습니다. 시아닌은 형광 염료로서, 특히 생체 의학 이미징에서 많은 용도로 사용되며 구조에 따라 IR에서 UV까지 스펙트럼을 커버합니다.

Cyanine은 polymethine 그룹에 속하는 합성 염료 계열의 비 계통적 이름입니다. cyanin이라는 단어는 일반적으로 청록색 ( “아쿠아”에 가까운)의 그늘을 의미하는 영어 단어 “cyan”에서 유래되었으며 다소 다른 색상 “진한 파란색 “을 의미하는 그리스어 κυάνεος / κυανοῦς kyaneos / kyanous에서 파생되었습니다.

Cy7은 육안으로 보이지 않는 근적외선 (near-IR) 불소로서 (여기 / 방출 최대 750/776 ) Cy7.5 염료뿐만 아니라 생체 내 이미징 응용 프로그램에 사용됩니다.

Cyanines (Cy)는 Fluorescein (FITC) 및 rhodamines (TRITC, RRX)와 같은 기존의 염료를 대체하여 보다 밝고 안정한 형광을 생성합니다.

 

 

CFP

Spectrum Chart

Excitation () Emission ()
433 475

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
39001 435/20
(425~445)
455 (LP) 480/30
(465~495)
Narrow Excitation band,
Bandpass Emission
49001 436/20
(426~446)
455 (LP) 480/40
(460~500)
Narrow Excitation band,
Bandpass Emission
  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49902 436/20
(426~446)
442 (LP) 480/40
(460~500)
For use with laser between 430-445

Narrow Excitation band,
Bandpass Emission

49903 436/20
(426~4460)
442 (LP) 500/40
(480~520)
For use with laser between 430-445

Narrow Excitation band,
Bandpass Emission

Information

Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 laser line and is optimally detected at 485 .

시안 형광 단백질 (Cyan Fluorescent Protein, CFP)은 생리 학적 과정을 모니터링하고, 단백질의 위치를 시각화하며, 생체 내에서 유전자 변형 (transgenic expression)을 검출하는 다목적 생물학적 표지자로서 CFP는 405 nm 레이저 라인에 의해 여기 될 수 있으며 485 에서 최적으로 검출됩니다.

Cy5

Spectrum Chart

Cy5 Spectrum
Excitation () Emission ()
649 670

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R2(B) 620/50
(595~645)
655 (LP) 690/50
(665~715)
Wide Excitation band,
Bandpass Emission
49006 620/60
(590~650)
660 (LP) 700/75
(662.5~737.5)
Very Wide Excitation band,
Bandpass Emission
49009 640/30
(625~655)
660 (LP) 690/50
(665~715)
Medium Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49913 635/20
(625~645)
640 (LP) 655(LP) For use with laser between 627~643

Narrow Excitation band,
Longpass Emission

49914 642/20
(632~652)
647 (LP) 705/72
(669~741)
For use with laser between 633~650

Narrow Excitation band,
Bandpass Emission

Information

Cy5 dye is a bright, far-red-fluorescent dye that can be excited using the 633 or 647 laser lines, and visualized Cy5 filter sets.  In addition to immunocytochemistry applications, Cy5 is also commonly used to label nucleic acids.

Cy5 염료는 633 또는 647 레이저 라인을 사용하여 여기되고 Cy5 필터 세트를 시각화 할 수있는 밝은 원색 – 형광 염료로서 면역 세포 화학 응용뿐만 아니라 핵산 표지에도 일반적으로 사용됩니다.

 

 

AlexaFluor 594

Spectrum Chart

Alexa Fluor 594 Spectrum
Excitation () Emission ()
590 617

 

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-R1(B) 560/40
(540~580)
600 (LP) 635/60
(605~665)
Wide Excitation band,
Bandpass Emission
49008 560/40
(540~580)
600 (LP) 630/75
(592.5~667.5)
Wide Excitation band,
Bandpass Emission
49306 580/25
(567.5~592.5)
600 (LP) 625/30
(610~640)
Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49911 594/10
(589~599)
594 (LP) 645/75
(607.5~682.5)
Narrow Excitation band,
Bandpass Emission

 

Information

Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 or 594 laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range.

Alexa Fluor® 594 염료는 561  또는 594 ㎚ 레이저 라인을 사용하여 여기 될 수있는 밝은 적색 형광 염료입니다. 이미징 및 유동 세포 계측법에서 안정적인 신호 생성을 위해 Alexa Fluor® 594 염료는 넓은 molar 농도 범위에서 pH에 둔감합니다.

 

 

TRITC

 

Spectrum Chart

TRITC Spectrum
Excitation () Emission ()
555 580

 

 

Recommended Filter set

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-G(B) 540/25
(527.5~552.5)
565 (LP) 605/55
(577.5~632.5)
Narrow Excitation band,
Bandpass Emission
49004 545/25
(532.5~557.5)
565 (LP) 605/70
570~640)
Narrow Excitation band,
Bandpass Emission
49305 546/22
(535~557)
565 (LP) 590/33
(573.5~606.5)
Narrow Excitation band,
Bandpass Emission

  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49908 532/10
(527~537)
532 (LP) 590/50
(565~615)
For use with laser between 529~535

Very Narrow Excitation band,
Bandpass Emission

49909 561/10
(556~566)
561 (LP) 600/50
(575~625)
For use with laser between 557~563

Narrow Excitation band,
Bandpass Emission

Information

Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

Tramethylrhodamine (TRITC)은 532 ㎚ 레이저 라인에 이상적으로 적합한 여기가있는 밝은 주황색 형광 염료로서 일반적으로 세포 이미징 응용을위한 항체와 단백질에 접합됩니다.

 

FITC

Spectrum Chart

FITC Spectrum
Excitation () Emission ()
490 525

 

Recommended Filter

  • 일반 형광 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-B(B) 480/30
(465~495)
505 (LP) 535/40
(515~555)
Medium Excitation band,
Bandpass Emission
49002 470/40
(450~490)
495 (LP) 525/50
(500~550)
Wide Excitation band,
Bandpass Emission
49011 480/40
(460~500)
510 (LP) 535/50
(510~560)
Wide Excitation band,
Bandpass Emission
  • 레이저 Lighting source 사용시

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
49904 488/10
(483~493)
488 (LP) 525/50
(500~550)
For use with laser between 485~491

Very Narrow Excitation band,
Bandpass Emission

Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluoresce in molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.

A succinimidyl-ester functional group attached to the fluorescein core, creating “NHS-fluorescein”, forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles.

FITC has excitation and emission spectrum peak wavelengths of approximately 495 ㎚ /519 . Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.

Fluorescein isothiocyanate (FITC)는 유동 세포 계측법을 포함한 광범위한 응용 분야에서 사용되는 fluorescein의 유도체입니다. FITC는 이소 티오 시아 네이트 반응기 (-N = C = S)로 관능화된 원래의 플루오 레세 인 분자로 구조의 바닥 링상의 수소 원자를 대체합니다. 이 유도체는 단백질상의 아민 및 설프 하이 드릴 그룹을 포함하는 친핵체에 대한 반응성입니다.

“NHS- 플루 오레 신”을 생성하는 플루오 레세인 코어에 부착된 숙신 이미 딜 – 에스테르 작용기는 다른 친핵체 존재하에서 제 1 급 아민에 대해 훨씬 더 큰 특이성을 갖는 또 다른 통상적 인 아민 반응성 유도체를 형성합니다.

FITC는 약 495 / 519 ㎚ 의 여기 및 방출 스펙트럼 피크 파장을 갖습니다. 대부분의 fluorochromes 처럼, 그것은 photobleaching하는 경향이 있습니다. photobleaching 의 문제로 인해 Alexa 488과 DyLight 488과 같은 fluorescein의 유도체는 광 안정성, 형광 강도 또는 다른 부착 그룹이 필요한 다양한 화학 및 생물학적 응용 분야에 맞춰졌습니다.


If you need more information for recommended filter sets, please click the model No. If you can’t click it, please contact us.

DAPI

Spectrum Chart

DAPI Spectrum
Excitation () Emission (㎚)
359 461

 

Recommended Filter set

  • 일반 형광 Lighting source

Filter Model No. Excitation filter (㎚) Dichroic mirror (㎚) Emission filter (㎚) Remarks
JNO-U(B) 375/28
(361-389)
415 (LP) 460/50
(435-485)
Narrow Excitation band,
Bandpass Emission
49000 350/50
(325-375)
400 (LP) 460/50
(435-485)
Wide Excitation band,
Bandpass Emission
49028 395/25
(382.5-407.5)
425 (LP) 460/50
(435-485)
For 395㎚ light sources

Narrow Excitation band,
Bandpass Emission

 

  • 레이저 Lighting source -> 문의 주시기 바랍니다.

 

 

Information

DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.

DAPI (4 ‘, 6-diamidino-2-phenylindole)는 DNA의 A-T가 풍부한 부위에 강하게 결합하는 형광 염료입니다. 그것은 형광 현미경에서 광범위하게 사용됩니다. DAPI는 손상되지 않은 세포막을 통과 할 수 있기 때문에 살아있는 세포에서 덜 효율적으로 막을 통과하기 때문에 살아있는 세포와 고정 된 세포를 모두 염색하는 데 사용할 수 있으므로 얼룩 효과는 낮습니다.

AcquCAM 23S

AcquCAM 23S

(Down load Catalog ACQUCAM 23S)

  • USB 3.0 Monochrome Camera
  • 1/1.2 ” Exmor Sensor
  • 1920×1200 pixel
  • Trigger input and I/O

AcquCAM 23S
EXMOR Sensors: Next-generation imaging sensors based on CMOS, increase the strength and reduce noise.

 

High sensitivity camera available from visible to IR range

20151120_1(CA1 pyramidal neuron)
Apply high sensitivity EXMOR sensor with additional filter block visible range, IR Cut filter image could be observed.

 

Available wider range of observation

AcquCAM 23S_6
By applying a 1 / 1.2 ” EXMOR sensor, you can observe a wider range of superior sensitivity image.
These images were shot by AcquCAM 23S Package.

High sensitivity and fast frame image

AcquCAM 23S_(CA3 pyramidal neuron)

AcquCAM 23S_(Dentate gyrus Granule cell)
To maximize the range of imaging possible observation with fast frame 54Fps and high sensitivity 0.015 lx .

[Specification]

Spec Description
Size 1/1.2 inch
Resolution H: 1920, V: 1200
Pixel size H: 5.86 µm, V: 5.86 µm
Video formats

& Frame rate

1920×1200 Y16 @ 25 fps

1920×1200 Y800 @ 54 fps

Sensitivity 0.015 lx
Exposure time 1/100000 to 30 s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65 g

 

AcquCAM 23G

AcquCAM 23G

(Down load Catalog ACQUCAM 23G)

  • USB 3.0 Color Camera
  • 1/1.2 ” Exmor Sensor
  • 1920×1200 pixel
  • Trigger input and I/O

AcquCAM 23G

The displays live digital images with gradual smoothness and combines exceptional resolution  with faithful color reproduction.The new sensors feature a global shutter function and able to capture a high-speed moving image without focal plane distortion. High-speed processing, low noise and low power dissipation by using column-parallel A/D conversion. equipped with trigger mode, and the external pulse can control accumulation time. The Sensor also have a pulse output function to indicate respective conditions during shutter operation and can be coordinated with peripheral circuits. High-definition images can be displayed live at a rate of high frames per second. without compression. Such imaging quality enables even the finest cellular regions to be observed clearly and distinctly without deterioration. While focusing is made stress free.

Universal camera- wide range of image acquisition  is available

Setting function of variety color images

AcquCAM23_16
With wide range of color temperature setting function, you can obtain a clear image of high sensitivity in accordance with the characteristics of samples you want to observe.

 

Live sharpness adjustment

You can observe and obtain sharp images by applying the sharpness function even when the live video output
You can observe and obtain sharp images by applying the sharpness function even when the live video output

 

 

[Specification]
Spec Description
Size 1/1.2 inch
Resolution H: 1920, V: 1200
Pixel size H: 5.86 µm, V: 5.86 µm
Video formats

& Frame rate

1920×1200 RGB32 @ 54 fps

1920×1200 Y16 @ 25 fps

1920×1200 Y800 @ 54 fps

Dynamic range 8 / 12 bit
Exposure time 1/100000 to 30 s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass

65 g