Example Configurations for Materials Science and Industry

BX53M Reflected and Reflected/Transmitted Light Combination

There are two types of microscope frames in the BX3M series, one for reflected light only and one for both reflected and transmitted light. Both frames can be configured with manual, coded, or motorized components. The frames are outfitted with ESD capability to protect electronic samples.

BX53M IR Combination


IR objectives can be used for semiconductor inspection, measurement, and processing applications where imaging through silicon is required to see the pattern. 5X to 100X infrared (IR) objectives are available with chromatic aberration correction from visible light wavelengths through the near infrared. For highmagnification work, rotating the correction collar of the LCPLNIR series of lenses corrects for aberrations caused by sample thickness. A clear image is obtained with a single objective.

BX53M Polarized Light Combination

The optics of the BX53M polarized light combination provide geologists with the right tools for high-contrast polarized light imaging. Applications such as mineral identification, investigating the optical characteristics of crystals, and observing solid rock sections benefit from system stability and precise optical alignment.

Bertrand Lens for conoscopic and orthoscopic observations

With a U-CPA conoscopic observation attachment, switching between orthoscopic and conoscopic observation is simple and fast. It is focusable for clear back focal plane interference patterns. The Bertrand field stop makes it possible to obtain consistently sharp and clear conoscopic images.

An Extensive Range of Compensator and Wave Plates

Six different compensators are available for measurements of birefringence in rock and mineral thin sections. Measurement retardation level ranges from 0 to 20λ. For easier measurement and high image contrast, the Berek and Senarmont compensators can be used, which change the retardation level in the entire field of view.

Measuring range of compensators

ompensator Measuring Range Major Application
Thick Berek(U-CTB) 0-11,000 nm
Measurement of High Retardation Level (R*>3λ), (crystals, macromolecules, fiber, etc.)
Berek(U-CBE) 0-1,640 nm
Measurement of Retardation Level (crystals, macromolecules, living organisms, etc.)
SenarmontCompensator(U-CSE) 0-546 nm
Measurement of Retardation Level (crystals, living organisms, etc.) Enhancement of Image Contrast (living organisms, etc.)
Brace-Koehler Compensator
1/10λ (U-CBR1)
0-55 nm
Measurement of Low Retardation Level (living organisms, etc.)
Enhancement of Image Contrast (living organisms, etc.)
Brace-Koehler Compensator
1/30λ (U-CBE2)
0-20 nm
Measurement of image contrast
(living organisms, etc.)
Quartz Wedge(U-CWE2) 500-2,200 nm
Approximate Measurement of Retardation Level (crystal, macromolecules, etc.)

*R = retardation level

For more accurate measurement, it is recommended that compensators (except U-CWE2) be used together with the interference filter 45-IF546.

Strain Free Optics

Thanks to Olympus’ sophisticated design and manufacturing technology, the UPLFLN-P strainfree objectives reduce internal strain to the minimum. This means a higher EF value, resulting in excellent image contrast.

BXFM System

The BXFM can be adapted to special applications or integrated into other instruments. The modular construction provides for straightforward adaptation to unique environments and configurations with a variety of special small illuminators and fixturing mounts.

Modular Design, Build Your System Your Way

Microscope Frames

There are two microscope frames for reflected light, one also has transmitted Light; capability. An adapter is available to raise the illuminator to accommodate taller samples.


■: Possible Reflected light Transmitted light Sample height
1 BX53MRF-S 0-65 mm
2 BX53MTRF-S 0-35 mm
3 BX3M-ARMAD +40 mm


For microscopy applications where the sample will not fit on a stage, the illuminator and optics can be mounted to a larger stand or to another piece of equipment.



BXFM + BX53M illuminator configuration

1 BXFM-F Frame interface is wall mounting/32 mm pillar
2 BX3M-ILH Illuminator holder
3 BXFM-ILHSPU Counter spring for BXFM
6 SZ-STL Large stand

Unit Combination Required: (1, 2 and 6)
Option: 3

BXFM + U-KMAS illuminator configuration

1 BXFM-F Frame interface is wall mounting/32 mm pillar
5 U-ST Stand
6 SZ-STL Large stand

Unit Combination Required: (1, 4 and 5) or (1, 4 and 6)
Option: –


For microscope imaging with eyepieces or for camera observation, select tubes by imaging type and operator’s posture during observation.

  FN Type Angle type Image Number of diopter 

adjustment mechanisms

1 U-BI30-2 22 Binocular Fixing Reverse 1
2 U-TBI-3 22 Binocular Tilting Reverse 1
3 U-TR30-2 22 Trinocular Fixing Reverse 1
4 U-TR30IR 22 Trinocular for IR Fixing Reverse 2
5 U-ETR-4 22 Trinocular Fixing Erect 2
6 U-TTR-2 22 Trinocular Tilting Reverse 2
7 U-SWTR-3 26.5 Trinocular Fixing Reverse 2
8 U-SWETTR-5 26.5 Trinocular Tilting Erect 2
9 U-TLU 22 Single port
10 U-TLUIR 22 Single port for IR


The illuminator projects light onto the sample based on the observation method selected. Software interfaces with coded illuminators to read the cube position and automatically recognize the observation method.


■: Possible Coded function Light source BF DF DIC POL IR FL MIX AS


1 BX3M-RLAS-S Fixed 3 cube position LED – Built in
2 BX3M-URAS-S Attachable 4 cube position LED

/Light guide

4 BX3M-KMA-S   LED – Built in
5 BX3-ARM Mechanical arm

for transmitted light


Light Sources

Light sources and power supplies for sample illumination, choose the appropriate light source for the observation method.



Standard LED light resource configuration

1 BX3M-LEDR LED lamp housing for reflected light
2 U-RCV DF converter for BX3M-URAS-S, required for observation with DF and BF when necessary
3 BX3M-PSLED Power supply for LED lamp housing, requires BXFM system
4 BX3M-LEDT LED lamp housing for transmitted light

Unit Combination Required: (1 and 3) or 4*1
Option: 2
*1:Only using BX53MTRF-frame

Fluorescence standard light resource configuration

5 U-LLGAD Light guide adapter
2 U-RCV DF converter for BX3M-URAS-S, required for observation
with DF and BF when necessary
6, 7 U-LLG150 (300) Light guide, length:1.5 m (3 m)
8 U-HGLGPS Light source for fluorescence

Unit Combination Required: (5, 6 and 8) or (5, 7 and 8)
Option: 2

Fluorescence mercury light resource configuration

9, 10 U-LH100HG(HGAPO) Mercury lamp housing for fluorescence
2 U-RCV DF converter for BX3M-URAS-S, required for observation with DF and BF when necessary
11 U-RFL-T Power supply for 100W mercury lamp

Unit Combination Required: (9 and 11) or (10 and 11)
Option: 2

Halogen and Halogen IR light resource configuration

12 U-LH100L-3 Halogen lamp housing
13 U-LH100IR Halogen lamp housing for IR
14 U-RMT Extender cable for halogen lamp housing, cable length 1.7 m (requires cable extension when necessary)
15, 16 TH4-100 (200) 100V (200V) specification power supply for 100W/50W halogen lamp
17 TH4-HS Hand switch for light intensity of halogen (dimmer TH4-100 (200) without hand switch)

Unit Combination Required: (12 and 15) or (12 and 16) or (13 and 15) or (13 and 16)
Option: 14, 17


Attachment for objectives and sliders. Select by the number of objectives needed and types; also with/without slider attachment.



■: Possible Type Holes BF DF EIC MIX ESD Number of centering holes
1 U-P4RE Manual 4         4
2 U-5RE-2 Manual 5          
3 U-5RES-ESD Coded 5        
4 U-D6RE Manual 6        
5 U-D6RE-ESD-2 Manual 6      
6 U-P6RE Manual 6       2
7 U-D7RE Manual 7        
8 U-D6RES Coded 6        
9 U-D7RES Coded 7        
10 U-D5BDREMC Motorized 5    
11 U-5BDRE Manual 5        
12 U-D5BDRE Manual 5    
13 U-P5BDRE Manual 5   2
14 U-D6BDRE Manual 6    
15 U-D5BDRES-ESD Coded 5  
16 U-D6BDRES-S Coded 6  
17 U-D6REMC Motorized 6    
18 U-D6BDREMC Motorized 6    


Select the slider to compliment traditional brighfield observation. The DIC slider provides topographic information about the sample with options to maximize contrast or resolution. The MIX slider provides illumination flexibility with a segmented LED source in the darkfield path.

  Type Amount of shear Available objectives
2 U-DICRH Resolution Small MPLFLN, MPLAPON

MIX slider for MIX observation.

  Type Available objectives

Controls Box and Hand Switches

Control boxes for interfacing microscope hardware with a PC and hand switches for hardware display and control.



BX3M-CB (CBFM) configuration

1 BX3M-CB Control box for BX53M system
2 BX3M-CBFM Control box for BXFM system
3 BX3M-HS MIX observation control, indicator of coded hardware, programmable function button of software (Stream)
4 BX3M-HSRE Motorized nosepiece rotation
5 U-HSEXP Shutter operation of camera

Unit Combination Required: 1 or 2
Option: 3, 4, 5

U-CBS configuration

6 U-CBS Control box for coded functions in BXFM configuration
5 U-HSEXP Shutter operation of camera

Unit Combination Required: 6
Option:  5


U-MIXRCBL (ECBL) U-MIXR cable, cable length: 0.5 m (2.9 m)
  BX3M-RMCBL (ECBL) Motorized nosepiece cable, cable length: 0.2 m (2.9 m)


Stages and stage plates for sample placement. Select based on sample shape and size.



150 mm × 100 mm stage configuration

1 U-SIC64 150 mm × 100 mm flat top handle stage
2 U-SHG (T) Silicone rubber operability handle rubber for improvement (thick type)
3 U-SP64 Stage plate for U-SIC64
4 U-WHP64 Wafer plate for U-SIC64
5 BH2-WHR43 Wafer holder for 4-3 in.
6 BH2-WHR54 Wafer holder for 5-4 in.
7 BH2-WHR65 Wafer holder for 6-5 in.
8 U-SPG64 Glass plate for U-SIC64

Unit Combination
Required: (1 and 3) or (1 and 8) or (1, 4 and 5) or (1, 4, and 6) or (1, 4 and 7*1)
Option: 2
*1:Only using U-WHP64 unit

100 mm × 100 mm stage configuration

9, 10 U-SIC4R (L) 2 105 mm × 100 mm right (left) handle stage
11 U-MSSP4 Stage plate for U-SIC4R (L) 2
12 U-WHP2 Wafer plate for U-SIC4R (L) 2
6 BH2-WHR43 Wafer holder for 4-3 in.
13 U-MSSPG Glass plate for U-SIC4R

Unit Combination
Required: (9 and 11) or (9 and 12) or (9, 11 and 12) or (10 and 11) or (10 and 13) or (10, 11 and 12)
Option: –

76 mm × 52 mm stage configuration

14, 15 U-SVR (L) M 76 mm × 52 mm right (left) handle stage
2 U-SHG (T) Silicone rubber operability handle rubber for improvement (thick type)
16 U-MSSP Stage plate for U-SVR (/L) M
17, 18 U-HR (L) D-4 Thin slide holder for the right (left) opening
19, 20 U-HR (L) DT-4 Thick slide holder for the right (left) opening, for pressing the slide glass
to stage top surface, the specimen is difficult to lift.

Unit Combination
Required: (14, 16, 17 and 19) or (14, 16, 17 and 20) or (14, 16, 18 and 19) or (14, 16, 18 and 20) or (15, 16, 17 and 19) or (15, 16, 17 and 20) or (15, 16, 18 and 19) or (15, 16, 18 and 20)
Option: 2

Rotatable stage configuration

21 U-SRG2 Rotatable stage
22 U-SRP Rotatable stage for POL, from any position can be 45° click stop
23 U-FMP Mechanical stage for U-SRP/U-SRG2

Unit Combination
Required: (21 and 24) or (22 and 24)
Option: 23

Fixed stage configuration

1 U-SP Fixed stage of a single plate

Camera Adapters

Adapter for camera observation. Selectable from required field of view and magnification. Actual observation range can be calculated using this formula:
actual field of view (diagonal mm) = viewing field (viewing number) ÷ objective magnification.

  Magnification Centering adjustment CCD image area (field number)
2/3 inch 1/1.8 inch 1/2 inch
1 U-TV1x-2 with U-CMAD3 1 10.7 8.8 8
2 U-TV1xC 1 ø2 mm 10.7 8.8 8
3 U-TV0.63xC 0.63 17 14 12.7
4 U-TV0.5xC-3 0.5 21.4 17.6 16
5 U-TV0.35xC-2 0.35 22
6 U-TV0.25xC 0.25


Eyepiece for viewing directly into the microscope. Select based on desired field of view.



■: Possible FN Diopter adjustment mechanism Built-in cross reticle
1 WHN10x 22    
2 WHN10x-H 22  
3 CROSS WHN10x 22
4 SWH10x-H 26.5  
5 CROSS SWH10x 26.5

Optical Filters

Optics filters convert sample exposure light to various types of illumination. Select the appropriate filter for observation requirements.



BF, DF, FL       1, 2, 3 U-25ND50, 25,6 Neutral density filter, transmittance 50%, 25%, 6%
4 U-25LBD Daylight color filter
5 U-25LBA Halogen color filter
6 U-25IF550 Green filter
7 U-25L42 UV-cut filter
8 U-25Y48 Yellow filter
9 U-25FR Frost filter (Required for the BX3M-URAS-S)
POL, DIC      10 U-AN-2 Polarization direction is fixed
11 U-AN360-3 Polarization direction is rotatable
12 U-AN360P-2 High quality polarization direction is rotatable
13 U-PO3 Polarization direction is fixed
14 U-POTP3 Polarization direction is fixed, for use with U-DICRH
15 45-IF546 Green ø45mm filter for POL
IR    16 U-AN360IR IR polarization direction is rotatable (reduces halation at IR observation when using combination with U-AN360IR and U-POIR)
17 U-POIR IR polarization direction is fixed
18 U-BP1100IR Band pass filter: 1100 nm
19 U-BP1200IR Band pass filter: 1200 nm
Transmitted light*  20 43IF550-W45 Green ø45 mm filter
21 U-POT Polarizer filter
Other  22 U-25 Empty filter, for use with user’s ø25 mm filters
23 U-FC Transmitted filter cassette; used by combining o45 mm filters

*AN and PO are not necessary when using BX3M-RLAS-S and U-FDICR


Condensers collect and focus transmitted light. Use for transmitted light observation.



1 U-AC2 Abbe condenser (available from 5x objectives)
2 U-SC3 Swing-out condenser (available from 1.25x objectives)
3 U-LWCD Long working distance condenser for glass plate (U-MSSPG, U-SPG64)
4 U-POC-2 Swing-out condenser for POL

Mirror Units

Mirror unit for BX3M-URAS-S. Select the unit for required observation.



1 U-FBF For BF, detachable ND filter
2 U-FDF For DF
3 U-FDICR For POL, crossed nicol position is fixed
4 U-FBFL For BF, built-in ND filter (It is necessary to use both BF* and FL)
*For coaxial episcopic illumination only
5 U-FWUS For Ultra Violet-FL BP330-385 BA420 DM400
6 U-FWBS For Blue-FL BP460-490 BA520IF DM500
7 U-FWGS For Green FL BP510-550 BA590 DM570
8 U-FF Empty mirror unit, Used user’s optical element

Intermediate Tubes

Various types of accessories for multiple purposes. For use between tube and illuminator.



1 U-CA Magnification changer (1x, 1.25x, 1.6x, 2x)
2 U-ECA Magnification changer (1x, 2x)
3 U-EPA2 Eye point adjuster: + 30 mm
4 U-DP Dual port for U-DP1xC
5 U-DP1xC C-mount TV camera adapter for U-DP
6 U-TRU Trinocular intermediate unit

UIS2 Objectives

Objectives magnify the sample. Select the objective that matches the working distance, resolving power and observation method for the application.

> Click here for details about UIS2 objective lenses